Can the Intel® Max® 10 Dual Configuration Intel FPGA IP Core trigger read operation from multiple registers in one operation from offset 2?

Yes.  In the Intel® Max® 10 Dual Configuration Intel FPGA IP Core, you need to trigger the desired operation from offset 2 before any read operation of offset 4, 5, 6, and 7.  Offset 2 has 4 bits to trigger read operation from 4 registers.  These bits are not one-hot.  So you can enable multiple bits in offset 2 to trigger read operations from multiple registers.

Please note that the time until ubusy de-assertion after offset 2 operation becomes longer as the number of registers to be read increases.

Refer to table 36 in Intel® MAX® 10 FPGA Configuration User Guide.