No. In Cyclone® V devices, the Active Serial (AS) pins DCLK, AS_DATA0, AS_DATA1, AS_DATA2, AS_DATA3 and nCSO have 25 kOhm pull-up resistors enabled only when the MSEL pins are set to AS configuration scheme. In Intel® Quartus® Prime software standard edition version 18.1 and earlier, the fitter report incorrectly says these pins have weak pull-up resistors regardless of configuration scheme in Device and Pin Options. The Cyclone V Device Handbook: Volume 1: Device Interfaces and Integration version 2018.11.23 or earlier doesn't mention about pull-up resistors on these pins.
Ignore the fitter report which says these pins have weak pull-up resistors when AS configuration scheme is not chosen in Intel Quartus Prime software.
A future release of the Cyclone V Device Handbook: Volume 1: Device Interfaces and Integration will be updated ti mention that these pins have 25 kOhm pull-up resistors when the MSEL pins are set to AS configuration scheme.